the n-terminal amino acid sequence of deduced mature protease is identical to that of the purified alkaline protease 但與其它絲氨酸蛋白酶基因相比,則同源性低于60%。
the result show that the ten n-terminal amino acids sequences of xyna are same as those of fxyn published by atsushi 將xyna1-w序列克隆進(jìn)酵母表達(dá)載體上,在畢赤酵母gs115中得到高效特異性表達(dá)。
based on the high homologous n-terminal amino acid sequence between ba-dfe and subtilisin bpn, the fragment encoding ba-dfe mature peptide was amplified from the total dna of b . amyloliquefaciens dc-4 by pcr 根據(jù)ba-dfe的n-端序列與subtilisinbpn高度同源設(shè)計引物,pcr擴(kuò)增出ba-dfe成熟肽編碼區(qū)片段。
the purification of this halocin was achieved by combination of tangential flow filtration ( tff ), sephadex g50 and deae-sepharose chromatography . the n-terminal amino acid sequence was also determined by edman degradation halc8對大多數(shù)的極端嗜鹽古菌(包括部分嗜鹽堿古菌)有抑制作用,但對細(xì)菌則無明顯的抑制作用。
sequencing and genbank blasting demonstrated that these four had the same sequences and encoded part of arabidopsis choloroplast carbonic anhydrase ( ca ) . the coding region contained 251 amino acid, from n terminal amino acid 86 to c terminus 到genbank進(jìn)行序列比對,結(jié)果表明它們編碼擬南芥葉綠體碳酸酐酶(ca)基因,編碼區(qū)含有部分ca序列,從n端第86位氨基酸至c末端,共251個氨基酸。